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#How to use a eps file in flexi 12. pdf

A figure panel should not include composite images of bands originating from different blots, exposures, or gels.

Each figure should include all relevant controls, and when relevant, control samples should be run on the same blot or gel as the experimental samples.

When a comparative analysis amongst bands is performed, all relevant samples should be run on the same gel/blot.

Any non-specific bands from the original image should be included in the figure and explained in the text or figure legend. Figure panels should include some background area above and below bands.

Images in figures should not be overcropped around the bands of interest.

Any adjustments to improve visibility of the data must be consistently applied to the entire image. The background and contrast should not be modified to notably alter the visibility of data, background, or non-specific bands.

In accordance with our Policy on Figure Preparation, the images should not be adjusted in any way that could affect the scientific information displayed.

Reporting blot and gel resultsĪuthors must comply with the following guidelines when preparing figures that report results from blots (including, but not limited to, western blots) and electrophoretic gels: If concerns are noted after publication, journal staff will follow up with authors and correct the literature as needed. A manuscript must comply with the above requirements, and the guidelines below, in order to be published. If any concerns arise about the availability of the original blot or gel images or compliance with the figure preparation guidelines (see below), the submission will be placed on hold while this is resolved.

#How to use a eps file in flexi 12. pdf

If your PDF size is larger, please use a suitable repository or discuss with the journal staff. Please note, there is a 20 MB maximum file size.All labeling and annotation should be performed without obscuring any data or background bands. Molecular weight markers should be included or indicated on the raw image, and any lanes not included in the final figure should be marked with an “X” above the lane label on the original blot/gel image. Authors should label each raw blot or gel image to clearly annotate the loading order, identity of experimental samples, method used to capture the image, and to specify which figure panel was generated from that original image.The file should be named ‘S1_raw_images’ and uploaded as a Supporting Information file or deposited at a suitable publicly-available data repository, with the dataset identifier (DOI or other form of persistent identifier) provided in the Data Availability Statement.We do not recommend compiling the images in Powerpoint as resolution can be lost. You could also use a PDF program to build a single PDF compiled from multiple annotated jpeg/tiff image files. Gimp, Photoshop) to compile and annotate the original images and then exporting/saving as a tiff file with LZW compression. Please create a single PDF file that contains all the original blot and gel images contained in the manuscript’s main figures and supplemental figures.Please follow these instructions when preparing and submitting blot/gel data files: Whilst it is not necessary to provide original images at time of initial submission, we will require these files during the peer review process or before a manuscript can be accepted. If you have questions about these requirements please email us at Original images for blots and gelsĪuthors must provide the original, uncropped and minimally adjusted images supporting all blot and gel results reported in an article’s figures and supporting information files. The figure preparation guidelines that follow clarify PLOS ONE standards and requirements, and aim to ensure the integrity and scientific validity of blot/gel data reporting. The original images also provide additional information for readers about background within the experiment and the specificity of reagents used. The underlying data requirement is in place to ensure that the results are reported in a fully transparent manner, and that readers can verify results by reviewing the primary data in its original form.

The following requirements apply to any figures and supporting Information files that report blot or gel data.